HORDB

General Information

ID: hor006612
Uniprot ID: P01211
Protein name: Synenkephalin
Gene name: PENK
Organism: Bos taurus (Bovine)
Family: Opioid neuropeptide precursor family
Source: Animal
Expression: Secreted by neuroendocrine chromaffin cells through cromaffin granules.
Disease: NA
Comments: NA
Taxonomy: Bos (genus), Bovinae (subfamily), Bovidae (family), Pecora (infraorder), Ruminantia (suborder), Artiodactyla (order), Laurasiatheria (superorder), Boreoeutheria, Eutheria, Theria, Mammalia (class), Amniota, Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
GO MF: GO:0001515 opioid peptide activity; GO:0031628 opioid receptor binding; GO:0048018 receptor ligand activity
GO BP: GO:0001662 behavioral fear response; GO:0001964 startle response; GO:0002118 aggressive behavior; GO:0007218 neuropeptide signaling pathway; GO:0007268 chemical synaptic transmission; GO:0007600 sensory perception; GO:0007626 locomotory behavior; GO:0009617 response to bacterium; GO:0019226 transmission of nerve impulse; GO:0019233 sensory perception of pain; GO:0038003 G protein-coupled opioid receptor signaling pathway; GO:0042742 defense response to bacterium
GO CC: GO:0005576 extracellular region; GO:0005886 plasma membrane; GO:0030425 dendrite; GO:0031410 cytoplasmic vesicle; GO:0034466 chromaffin granule lumen; GO:0043025 neuronal cell body; GO:0043679 axon terminus

Sequence Information

Sequence: ECSQDCATCSYRLARPTDLNPLACTLECEGKLPSLKTWETCKELLQLTKLELPPDATSALSKQEESHLLA
Length: 70(25-94)
Propeptide: MARFLGLCTWLLALGPGLLATVRAECSQDCATCSYRLARPTDLNPLACTLECEGKLPSLKTWETCKELLQLTKLELPPDATSALSKQEESHLLAKKYGGFMKRYGGFMKKMDELYPLEVEEEANGGEVLGKRYGGFMKKDAEEDDGLGNSSNLLKELLGAGDQREGSLHQEGSDAEDVSKRYGGFMRGLKRSPHLEDETKELQKRYGGFMRRVGRPEWWMDYQKRYGGFLKRFAEPLPSEEEGESYSKEVPEMEK
Signal peptide: MARFLGLCTWLLALGPGLLATVRA
Modification: NA
Glycosylation: NA
Mutagenesis: NA

Activity

Function: [Met-enkephalin]: Neuropeptide that competes with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress.
Mechanism: NA
Cross BBB: NA
Target: OPRL1
Target Unid: A0A3Q1LTY9
IC50: NA
EC50: NA
ED50: NA
kd: NA
Half life: NA

Structure

Disulfide bond: 2-24; 6-28; 9-41
Structure ID: AF-P01211-F1(AlphaFold_DB_ID)

Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)

hor006612_AF2.pdb

hor006612_ESM.pdb

Physical Information

Mass:	896781	Formula:	C332H544N88O111S6
Absent amino acids:	FIMV	Common amino acids:	L
pI:	4.5	Basic residues:	8
Polar residues:	22	Hydrophobic residues:	21
Hydrophobicity:	-36.43	Boman Index:	-11399
Half-Life:	1 hour	Half-Life Yeast:	30 min
Half-Life E.Coli:	>10 hour	Aliphatic Index	86.57
Instability Index:	5990.29	Extinction Coefficient cystines:	7365
Absorbance 280nm:	106.74

Literature

PubMed ID: 6276759
Title: Cloning and sequence analysis of cDNA for bovine adrenal preproenkephalin.
PubMed ID: 6173760
Title: Molecular cloning establishes proenkephalin as precursor of enkephalin-containing peptides.
PubMed ID: 6952189
Title: Partial characterization of the mRNA that codes for enkephalins in bovine adrenal medu
PubMed ID: 8654396
Title:
PubMed ID: 12869695
Title: